Journal article

Depletion of a Toxoplasma porin leads to defects in mitochondrial morphology and contacts with the endoplasmic reticulum

Natalia Mallo, Jana Ovciarikova, Erica S Martins-Duarte, Stephan C Baehr, Marco Biddau, Mary-Louise Wilde, Alessandro D Uboldi, Leandro Lemgruber, Christopher J Tonkin, Jeremy G Wideman, Clare R Harding, Lilach Sheiner

JOURNAL OF CELL SCIENCE | COMPANY BIOLOGISTS LTD | Published : 2021

Abstract

The voltage-dependent anion channel (VDAC) is a ubiquitous channel in the outer membrane of the mitochondrion with multiple roles in protein, metabolite and small molecule transport. In mammalian cells, VDAC protein, as part of a larger complex including the inositol triphosphate receptor, has been shown to have a role in mediating contacts between the mitochondria and endoplasmic reticulum (ER). We identify VDAC of the pathogenic apicomplexan Toxoplasma gondii and demonstrate its importance for parasite growth. We show that VDAC is involved in protein import and metabolite transfer to mitochondria. Further, depletion of VDAC resulted in significant morphological changes in the mitochondrion..

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Grants

Awarded by Biotechnology and Biological Sciences Research Council


Awarded by Wellcome Trust Investigator Award


Awarded by Wellcome Trust


Awarded by Royal Society


Awarded by National Health and Medical Research Council in Australia


Funding Acknowledgements

This work is supported by a Biotechnology and Biological Sciences Research Council grant [BB/N003675/1] and aWellcome Trust Investigator Award [217173/Z/19/Z] to L.S. L.S is a Royal Society of Edinburgh Personal Research Fellow. C.R.H. holds a Sir Henry Dale fellowship from the Wellcome Trust and Royal Society [213455/Z/18/Z] and a Lord Kelvin/Adam Smith (LKAS) Fellowship from the University of Glasgow. C.J.T. is supported by the National Health and Medical Research Council in Australia [GNT1183496]. M.-L.W. is supported by an Australian Research Training Program Scholarship. J.G.W. is supported by a start-up grant from the School of Life Sciences and College of Liberal Arts and Sciences at Arizona State University. Deposited in PMC for immediate release.