Journal article
The histone acetyltransferase HBO1 promotes efficient tip cell sprouting during angiogenesis
ZL Grant, PF Hickey, W Abeysekera, L Whitehead, SM Lewis, RCA Symons, TM Baldwin, D Amann-Zalcenstein, AL Garnham, GK Smyth, T Thomas, AK Voss, L Coultas
Development Cambridge | Published : 2021
DOI: 10.1242/DEV.199581
Abstract
Blood vessel growth and remodelling are essential during embryonic development and disease pathogenesis. The diversity of endothelial cells (ECs) is transcriptionally evident and ECs undergo dynamic changes in gene expression during vessel growth and remodelling. Here, we investigated the role of the histone acetyltransferase HBO1 (KAT7), which is important for activating genes during development and for histone H3 lysine 14 acetylation (H3K14ac). Loss of HBO1 and H3K14ac impaired developmental sprouting angiogenesis and reduced pathological EC overgrowth in the retinal endothelium. Single-cell RNA sequencing of retinal ECs revealed an increased abundance of tip cells in Hbo1-deficient retin..
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Awarded by Royal Melbourne Hospital Neuroscience Foundation
Funding Acknowledgements
This work was supported by the National Health and Medical Research Council, Australia (Project Grants 1084248 and 1143612 to A.K.V. and T.T.; Research Fellowships 1081421 to A.K.V., 1154970 to G.K.S. and 1003435 to T.T.; Investigator Grant 1176789 to A.K.V.); the Australian Research Council (Future Fellowships: 110100891 to L.C.); the L.E.W. Carty Charitable Fund (L.C.); an Australian Government Research Training Program Scholarship (to Z.L.G.); and the Royal Melbourne Hospital Neuroscience Foundation (R.C.A.S.).