Journal article

Genomic knockout of hsp23 both decreases and increases fitness under opposing thermal extremes in Drosophila melanogaster

Xinyue Gu, Wei Chen, Trent Perry, Philip Batterham, Ary A Hoffmann

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2021

Abstract

Under exposure to harmful environmental stresses, organisms exhibit a general stress response involving upregulation of the expression of heat shock proteins (HSPs) which is thought to be adaptive. Small heat shock proteins (sHSPs) are key components of this response, although shsp genes may have other essential roles in development. However, the upregulation of expression of a suite of genes under stress may not necessarily be evidence of an adaptive response to stress that involves those genes. To explore this issue, we used the CRISPR/Cas9 system to investigate pleiotropic effects of the hsp23 gene in Drosophila melanogaster. Transgenic flies carrying a pCFD5 plasmid containing sgRNAs wer..

View full abstract