Development and Application of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Pseudomonas syringae Pathovars pisi and syringae
Pragya Kant, Mario Fruzangohar, Rachel Mann, Brendan Rodoni, Grant Hollaway, Garry Rosewarne
AGRICULTURE-BASEL | MDPI | Published : 2021
Pseudomonas syringae causes bacterial blight (BB) disease worldwide on economically important fruit and vegetable crops including field pea (Pisum sativum L.). The two pathovars responsible for BB in field pea are Pseudomonas syringae pathovar pisi (Psp) and syringae (Pss). In the field, both pathovars cause indistinguishable symptoms on field pea and require laboratory diagnosis to determine the causal pathovar. To aid in-field and laboratory diagnosis, accurate, and robust loop-mediated isothermal amplification (LAMP) assays for Psp and Pss were developed. The assays were able to detect Psp or Pss on live or heat-killed bacterial cells, plant exudates, seeds, and DNA extracts with no inhib..View full abstract
Awarded by Agriculture Victoria
This research received joined funding from Grains Research and Development Corporation (GRDC) and Agriculture Victoria under project "DAV1607-010BLX 9176093 PulseBio 3 Stable grain yield in pulses through improved stress tolerance".