Characterisation of the whole genome sequence of an avian hepatitis E virus directly from clinical specimens reveals possible recombination events between European and USA strains
Kinza Asif, Denise O'Rourke, Ahmad J Sabir, Pollob Shil, Amir H Noormohammadi, Marc S Marenda
INFECTION GENETICS AND EVOLUTION | ELSEVIER | Published : 2021
Avian hepatitis E virus (aHEV) is the causative agent of an important disease of broiler breeders and layers. aHEV cannot be readily propagated in cell culture and is characterised primarily by sequencing of amplicons generated through several RT-PCRs that target individual genes. This study aims to uncover the origin of current Australian aHEV isolates based on whole genome sequencing using clinical liver tissues. Complete genome sequences of the two aHEV isolates were assembled using Nanopore and Illumina reads. The two isolates possessed only four single nucleotide polymorphisms to each other. Comparison of the sequences with aHEV genome sequences available in the GenBank showed the highe..View full abstract
First author is a recipient of Melbourne research scholarship funded by the University of Melbourne, Melbourne, Australia. Internal funding for this study was provided by Asia Pacific Centre for Animal Health, the University of Melbourne.