Journal article

Molecular and genomic characterisation of a panel of human anal cancer cell lines

Glen R Guerra, Joseph C Kong, Rosemary M Millen, Matthew Read, David S Liu, Sara Roth, Shienny Sampurno, Joseph Sia, Maria-Pia Bernardi, Timothy J Chittleborough, Corina C Behrenbruch, Jiasian Teh, Huiling Xu, Nicole M Haynes, Jiaan Yu, Richard Lupat, David Hawkes, Natasha Di Costanzo, Richard W Tothill, Catherine Mitchell Show all

CELL DEATH & DISEASE | SPRINGERNATURE | Published : 2021

Abstract

Anal cancer is a rare disease that has doubled in incidence over the last four decades. Current treatment and survival of patients with this disease has not changed substantially over this period of time, due, in part, to a paucity of preclinical models to assess new therapeutic options. To address this hiatus, we set-out to establish, validate and characterise a panel of human anal squamous cell carcinoma (ASCC) cell lines by employing an explant technique using fresh human ASCC tumour tissue. The panel of five human ASCC cell lines were validated to confirm their origin, squamous features and tumourigenicity, followed by molecular and genomic (whole-exome sequencing) characterisation. This..

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Grants

Funding Acknowledgements

This work was supported by a National Health and Medical Research Council (NHMRC) of Australia Postgraduate Research Scholarship, Royal Australasian College of Surgeons (RACS) Tour De Cure Scholarship, RACS Foundation for Surgery Research Scholarship, RACS ANZ Journal of Surgery Scholarship, Covidien Colorectal Research Fellowship and Cancer Therapeutics Research Scholarship (Cancer Therapeutics CRC) to GRG.