Journal article

Poor protective potential of influenza nucleoprotein antibodies despite wide prevalence

Hillary A Vanderven, Robyn Esterbauer, Sinthujan Jegaskanda, Hyon-Xhi Tan, Adam K Wheatley, Stephen J Kent

IMMUNOLOGY AND CELL BIOLOGY | WILEY | Published : 2021

Abstract

Humans are exposed to influenza virus through periodic infections. Due to these repeated exposures, human populations commonly have elevated antibody titers targeting the conserved internal influenza virus nucleoprotein (NP). Despite the presence of anti-NP antibodies, humans are acutely susceptible to drifted influenza viruses with antigenically different surface proteins and the protective potential of human NP antibodies is unclear. In this study, high levels of anti-NP antibody and NP-specific B cells were detected in both adult humans and influenza-infected mice, confirming that NP is a major target of humoral immunity. Through sorting single B cells from influenza-exposed human adults,..

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Grants

Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

We sincerely thank all the healthy human donors who donated blood for this research. We also thank Dr Bruce Wines and Professor P Mark Hogarth (Burnet Institute, Melbourne, Australia) who provided the recombinant soluble Fc gamma R dimers and developed the Fc gamma R dimer ELISA. This work was supported by the Australian National Health and Medical Research Council (Award number 1052979 to SJK).