324. SLX4, A KEY REGULATOR OF MEIOSIS AND DNA REPAIR IN THE MALE GERMLINE

Abstract

Genome integrity relies on the ability of DNA repair proteins to open, cleave and recombine DNA. Slx4 (synthetic lethal of unknown function) or BTBd12 (BTBD12 domain-containing protein 12) the human homologue is a DNA repair protein which has multiple independent cellular roles. Importantly, it is a component in Holliday junction homologous recombination (HR) where it interacts with other proteins to repair double strand breaks (DSB). SLX4 forms a heterodimer complex with SLX1, an endonuclease, in response to DNA damage. This complex works by recruiting several associated proteins including XPF- ERCC1 a 3′ flap endonuclease, to DSB allowing proper single strand annealing repair of the DNA. A..