Journal article

Stability of Engineered Ferritin Nanovaccines Investigated by Combined Molecular Simulation and Experiments

Yiran Qu, Lijie Wang, Shuang Yin, Bingyang Zhang, Yan Jiao, Yan Sun, Anton Middelberg, Jingxiu Bi

JOURNAL OF PHYSICAL CHEMISTRY B | AMER CHEMICAL SOC | Published : 2021

Abstract

Human ferritin is regarded as an attractive and promising vaccine platform because of its uniform structure, good plasticity, and desirable thermal and chemical stabilities. Besides, it is biocompatible and presumed safe when used as a vaccine carrier. However, there is a lack of knowledge of how different antigen insertion sites on the ferritin nanocage impact the resulting protein stability and performance. To address this question, we selected Epstein-Barr nuclear antigen 1 as a model epitope and fused it at the DNA level with different insertion sites, namely, the N- and C-termini of ferritin, to engineer proteins E1F1 and F1E1, respectively. Protein properties including hydrophobicity a..

View full abstract

University of Melbourne Researchers