Journal article

Immunogenicity and Vaccine Efficacy Boosted by Engineering Human Heavy Chain Ferritin and Chimeric Hepatitis B Virus Core Nanoparticles

Yiran Qu, Bingyang Zhang, Yingli Wang, Shuang Yin, Yan Sun, Anton Middelberg, Jingxiu Bi

ACS APPLIED BIO MATERIALS | AMER CHEMICAL SOC | Published : 2021

Abstract

Human heavy-chain ferritin (HFn) and hepatitis B virus core (HBc) are both nanoparticle proteins presenting a well-oriented architecture with constant size and shape, which can be engineered to carry epitopes on the surface of the nanoparticle protein cage, enabling vaccine design. This study aims to investigate the immunogenicity differences between engineered HFn and chimeric HBc bearing the same epitope. As a proof of concept, the model epitope Epstein-Barr nuclear antigen 1 (EBNA1) is inserted at the N-terminus of the HFn and HBc subunit to produce two vaccine candidates named EBNA1-HFn (E1F1) and EBNA1-HBc (E1H1), respectively. From in vivo immunogenicity studies, E1H1 demonstrates the ..

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University of Melbourne Researchers