Journal article

Immobilization and Intracellular Delivery of Structurally Nanoengineered Antimicrobial Peptide Polymers Using Polyphenol-Based Capsules

Jiaying Song, Christina Cortez-Jugo, Steven J Shirbin, Zhixing Lin, Shuaijun Pan, Greg G Qiao, Frank Caruso

ADVANCED FUNCTIONAL MATERIALS | WILEY-V C H VERLAG GMBH | Published : 2021

Abstract

Structurally nanoengineered antimicrobial peptide polymers (SNAPPs) are an emerging class of antimicrobials against multidrug-resistant bacteria. Their encapsulation in particle carriers can improve their therapeutic efficacy by preventing peptide degradation, reducing clearance, and enhancing intracellular delivery and dosage to bacteria-infected host cells. Herein, two template-mediated strategies are reported for immobilizing SNAPPs in microcapsules through 1) complexation of SNAPPs with tannic acid (TA) onto porous CaCO3 templates and subsequent removal of the templates (SNAPP–TA capsules) and 2) adsorption of SNAPPs onto CaCO3 templates and subsequent encapsulation within a metal–phenol..

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Grants

Awarded by Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology


Awarded by National Health and Medical Research Council Senior Principal Research Fellowship


Funding Acknowledgements

This research was conducted and funded by the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036). F.C. acknowledges the award of a National Health and Medical Research Council Senior Principal Research Fellowship (GNT1135806). C.C.-J. acknowledges the award of a Melbourne Research Fellowship (The University of Melbourne). S.J.S. acknowledges the award of an Early Career Research Grant (Melbourne School of Engineering, The University of Melbourne). This work was performed in part at the Materials Characterization and Fabrication Platform (MCFP) at The University of Melbourne, the Victorian Node of the Australian National Fabrication Facility (ANFF), and the Biosciences Microscopy Facility at The University of Melbourne. The authors acknowledge Y. Han and J. Chen for assistance with experiments and helpful discussions.