Journal article
Sex- And tissue-specific effects of binge-level prenatal alcohol consumption on DNA methylation at birth
YJ Loke, E Muggli, R Saffery, J Ryan, S Lewis, EJ Elliott, J Halliday, JM Craig
Epigenomics | Published : 2021
Abstract
Background: Binge-level prenatal alcohol exposure (PAE) causes developmental abnormalities, which may be mediated in part by epigenetic mechanisms. Despite this, few studies have characterised the association of binge PAE with DNA methylation in offspring. Methods: We investigated the association between binge PAE and genome-wide DNA methylation profiles in a sex-specific manner in neonatal buccal and placental samples. Results: We identified no differentially methylated CpGs or differentially methylated regions (DMRs) at false discovery rate <0.05. However, using a sum-of-ranks approach, we identified a DMR in each tissue of female offspring. The DMR identified in buccal samples is located ..
View full abstractGrants
Awarded by Jack Brockhoff Foundation
Funding Acknowledgements
This work was supported by the Australian National Health and Medical Research Council Grant no. 1011070; Senior Research Fellowship no. 1021252 (J Halliday) and no. 1045161 (R Saffrey), and Practitioner Fellowship no. 1021480 (EJ Elliott); the Medical Research Futures Fund Next Generation Fellowship no. 1135959 (EJ Elliott); and the Victorian State Government's Operational Infrastructure Support Program. The study has also received funding from the McCusker Charitable Trust to assist with the biospecimen collection at birth, Jack Brockhoff Foundation no. 3525 for genome-wide DNA methylation data generation of placenta samples, and Foundation for Children no. 2015-111 for genome-wide DNA methylation data generation of buccal samples. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.