Journal article
Correlation of in vitro cell adhesion, local shear flow and cell density
AM Jötten, S Angermann, MEM Stamp, D Breyer, FG Strobl, A Wixforth, C Westerhausen
Rsc Advances | ROYAL SOC CHEMISTRY | Published : 2019
DOI: 10.1039/c8ra07416j
Abstract
Investigating cell adhesion behavior on biocompatible surfaces under dynamic flow conditions is not only of scientific interest but also a principal step towards development of new medical implant materials. Driven by the improvement of the measurement technique for microfluidic flow fields (scanning particle image velocimetry, sPIV), a semi-automatic correlation of the local shear velocity and the cell detachment probability became possible. The functionality of customized software entitled ‘PIVDAC' (Particle Image Velocimetry De-Adhesion Correlation) is demonstrated on the basis of detachment measurements using standard sand-blasted titanium implant material. A thermodynamic rate model is ..
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Awarded by Deutsche Forschungsgemeinschaft
Funding Acknowledgements
The authors thank the "Deutsche Forschungsgemeinschaft (DFG)" for the financial support of this work under the contract (WI 1091/19-1) "Erkenntnistransferprojekt Antibakterielle und abriebarme Beschichtung von Gleitflachen in orthopadischen Implantaten". Moreover, the authors like to acknowledge funding by Nanosystems Initiative Munich (NIM) and thank the Center for NanoScience (CeNS), the Bavaria California Technology Center (BaCaTec) and the Augsburg Centre for Innovative Technologies (ACIT) for additional financial support for this project. The authors thank Sidonie Lieber and Alexander Hartwig for technical assistance.