Journal article
Fragmentation of tissue-resident macrophages during isolation confounds analysis of single-cell preparations from mouse hematopoietic tissues.
Susan M Millard, Ostyn Heng, Khatora S Opperman, Anuj Sehgal, Katharine M Irvine, Simranpreet Kaur, Cheyenne J Sandrock, Andy C Wu, Graham W Magor, Lena Batoon, Andrew C Perkins, Jacqueline E Noll, Andrew CW Zannettino, David P Sester, Jean-Pierre Levesque, David A Hume, Liza J Raggatt, Kim M Summers, Allison R Pettit
Cell Rep | Published : 2021
Open access
Abstract
Mouse hematopoietic tissues contain abundant tissue-resident macrophages that support immunity, hematopoiesis, and bone homeostasis. A systematic strategy to characterize macrophage subsets in mouse bone marrow (BM), spleen, and lymph node unexpectedly reveals that macrophage surface marker staining emanates from membrane-bound subcellular remnants associated with unrelated cells. Intact macrophages are not present within these cell preparations. The macrophage remnant binding profile reflects interactions between macrophages and other cell types in vivo. Depletion of CD169+ macrophages in vivo eliminates F4/80+ remnant attachment. Remnant-restricted macrophage-specific membrane markers, cyt..
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