Journal article
Multi-year field evaluation of nicotianamine biofortified bread wheat
JT Beasley, JP Bonneau, LT Moreno-Moyano, DL Callahan, KS Howell, E Tako, J Taylor, RP Glahn, R Appels, AAT Johnson
Plant Journal | WILEY | Published : 2022
DOI: 10.1111/tpj.15623
Abstract
Conventional breeding efforts for iron (Fe) and zinc (Zn) biofortification of bread wheat (Triticum aestivum L.) have been hindered by a lack of genetic variation for these traits and a negative correlation between grain Fe and Zn concentrations and yield. We have employed genetic engineering to constitutively express (CE) the rice (Oryza sativa) nicotianamine synthase 2 (OsNAS2) gene and upregulate biosynthesis of two metal chelators – nicotianamine (NA) and 2′-deoxymugineic acid (DMA) – in bread wheat, resulting in increased Fe and Zn concentrations in wholemeal and white flour. Here we describe multi-location confined field trial (CFT) evaluation of a low-copy transgenic CE-OsNAS2 wheat e..
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Funding Acknowledgements
This work was supported by grants from the HarvestPlus program to AATJ and by the Albert Shimmins Fund to JTB. We thank Adrian Cox, Leigh Smith, Shahajahan Miyan, and Ed Barrett-Lennard at the Department of Primary Industries and Regional Development Western Australia (Western Australia, Australia) for excellent technical assistance related to CFT components of our research. We also thank Mary Bodis, Yongpei Chang, and Shree Giri for their invaluable help provided throughout the project. Analysis of NA and DMA was carried out at Metabolomics Australia (School of BioSciences, The University of Melbourne, Australia), an NCRIS initiative under Bioplatforms Australia Pty Ltd. The authors wish to dedicate this manuscript in memory of Adrian Cox.