Journal article
Spatio-temporal analysis of nanoparticles in live tumor spheroids impacted by cell origin and density
A Ahmed-Cox, E Pandzic, ST Johnston, C Heu, J McGhee, FM Mansfeld, EJ Crampin, TP Davis, RM Whan, M Kavallaris
Journal of Controlled Release | Published : 2022
Abstract
Nanoparticles hold great preclinical promise in cancer therapy but continue to suffer attrition through clinical trials. Advanced, three dimensional (3D) cellular models such as tumor spheroids can recapitulate elements of the tumor environment and are considered the superior model to evaluate nanoparticle designs. However, there is an important need to better understand nanoparticle penetration kinetics and determine how different cell characteristics may influence this nanoparticle uptake. A key challenge with current approaches for measuring nanoparticle accumulation in spheroids is that they are often static, losing spatial and temporal information which may be necessary for effective na..
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Awarded by Cancer Institute NSW
Funding Acknowledgements
This work was supported by the Children's Cancer Institute, which is affiliated with the University of New South Wales (UNSW Sydney), and the Sydney Children's Hospital Network, and by grants from the National Health and Medical Research (Program Grant APP1091261 and Principal Research Fellowship APP1119152 to MK) and Cancer Institute New South Wales Translational Program Grant (TPG2037 to MK). MK is also supported by Australian Research Council Center of Excellence in Convergent Bio-Nano Science and Technology (140100036CE). AA-C acknowledges support from the Scientia PhD Scholarship Scheme (UNSW Sydney), the Josee Hilton Excellence Award and Children's Cancer Institute Postgraduate Top-up Scholarship. STJ is supported by the Australian Research Council (DE200100988). The authors would like to thank the Katharina Gaus Light Microscopy Facility, at the Mark Wainwright Analytical Center for their support and resources involved in this work, and the Cancer Institute of NSW for a donation that allowed the acquisition of a Zeiss LSM 880 used in this work. The authors would also like to thank J. Fletcher for his mnemonic which refined the name of this analysis method.