Lung-resident memory B cells established after pulmonary influenza infection display distinct transcriptional and phenotypic profiles

HX Tan; JA Juno; R Esterbauer; HG Kelly; KM Wragg; P Konstandopoulos; S Alcantara; C Alvarado; R Jones; G Starkey; BZ Wang; O Yoshino; T Tiang; M Lindsay Grayson; H Opdam; R D'Costa; A Vago; LK Mackay; CL Gordon; D Masopust; JR Groom; SJ Kent; AK Wheatley

Journal article

Lung-resident memory B cells established after pulmonary influenza infection display distinct transcriptional and phenotypic profiles

HX Tan, JA Juno, R Esterbauer, HG Kelly, KM Wragg, P Konstandopoulos, S Alcantara, C Alvarado, R Jones, G Starkey, BZ Wang, O Yoshino, T Tiang, M Lindsay Grayson, H Opdam, R D'Costa, A Vago, LK Mackay, CL Gordon, D Masopust Show all

Science Immunology | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2022

DOI: 10.1126/sciimmunol.abf5314

Abstract

Recent studies have established that memory B cells, largely thought to be circulatory in the blood, can take up long-term residency in inflamed tissues, analogous to widely described tissue-resident T cells. The dynamics of recruitment and retention of memory B cells to tissues and their immunological purpose remains unclear. Here, we characterized tissue-resident memory B cells (BRM) that are stably maintained in the lungs of mice after pulmonary influenza infection. Influenza-specific BRM were localized within inducible bronchus-associated lymphoid tissues (iBALTs) and displayed transcriptional signatures distinct from classical memory B cells in the blood or spleen while showing partial ..

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University of Melbourne Researchers

Hyon Xhi Tan Author

Jennifer Juno Author

Robyn Esterbauer Author

Kathleen Wragg Author

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Grants

Awarded by NHMRC

Funding Acknowledgements

This study was supported by NHMRC program grant GNT1052979 (S.J.K.) and NHMRC project grants GNT1129099 and GNT1162760 (A.K.W.). Author contributions: H.-X.T., S.J.K., and A.K.W. designed the study, analyzed results, and wrote the manuscript. H.-X.T., J.A.J., R.E., H.G.K., K.M.W., P.K., S.A., and A.K.W. performed the experiments. D.M. established in situ probe staining procedures. C.A. and J.R.G. generated bone marrow CXCR3-/-/WT chimeras. R.J., G.S., B.Z.W., O.Y., T.T., M.L.G., H.O., R.D., A.V., L.K.M., and C.L.G. facilitated collection of human tissues. All authors read, edited, and approved the manuscript.