Journal article
Biofabrication of advanced in vitro 3D models to study ischaemic and doxorubicin-induced myocardial damage
P Sharma, C Liu Chung Ming, X Wang, LA Bienvenu, D Beck, G Figtree, A Boyle, C Gentile
Biofabrication | Published : 2022
Abstract
Current preclinical in vitro and in vivo models of cardiac injury typical of myocardial infarction (MI, or heart attack) and drug induced cardiotoxicity mimic only a few aspects of these complex scenarios. This leads to a poor translation of findings from the bench to the bedside. In this study, we biofabricated for the first time advanced in vitro models of MI and doxorubicin (DOX) induced injury by exposing cardiac spheroids (CSs) to pathophysiological changes in oxygen (O2) levels or DOX treatment. Then, contractile function and cell death was analyzed in CSs in control verses I/R and DOX CSs. For a deeper dig into cell death analysis, 3D rendering analyses and mRNA level changes of cardi..
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Awarded by University of Newcastle Australia
Funding Acknowledgements
Poonam Sharma was supported by University of Newcastle with UNIPRS and UNRS Central & Faculty School (UNRSC5050) scholarships. Laura A Bienvenu is supported by a National Heart Foundation (NHF) of Australia Postdoctoral Fellowship; Xiaowei Wang is supported by an NHF Future Leader Fellowship and a Baker Fellowship. Carmine Gentile was supported by a UTS Seed Funding, Catholic Archdiocese of Sydney Grant for Adult Stem Cell Research and a University of Sydney/Sydney Medical School Foundation Cardiothoracic Surgery Research Grant. A special thanks to Prof Louise Cole (UTS, Sydney) and to Dr Imala Alwis (Heart Research Institute, Sydney) for their assistance with confocal microscopy imaging.