Nanoscale probing and imaging of HIV-1 RNA in cells with a chimeric LNA-DNA sensor

A Amodio; M Cassani; L Mummolo; C Cortez-Jugo; SK Bhangu; J Symons; CL Ahlenstiel; G Forte; F Ricci; AD Kelleher; SR Lewin; F Cavalieri; F Caruso

Journal article

Nanoscale probing and imaging of HIV-1 RNA in cells with a chimeric LNA-DNA sensor

A Amodio, M Cassani, L Mummolo, C Cortez-Jugo, SK Bhangu, J Symons, CL Ahlenstiel, G Forte, F Ricci, AD Kelleher, SR Lewin, F Cavalieri, F Caruso

Nanoscale | ROYAL SOC CHEMISTRY | Published : 2022

DOI: 10.1039/d1nr08418f

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Abstract

Real-time detection and nanoscale imaging of human immunodeficiency virus type 1 ribonucleic acid (HIV-1 RNA) in latently infected cells that persist in people living with HIV-1 on antiretroviral therapy in blood and tissue may reveal new insights needed to cure HIV-1 infection. Herein, we develop a strategy combining DNA nanotechnology and super-resolution expansion microscopy (ExM) to detect and image a 22 base sequence transcribed from the HIV-1 promoter in model live and fixed cells. We engineer a chimeric locked nucleic acid (LNA)-DNA sensor via hybridization chain reaction to probe HIV-1 RNA in the U3 region of the HIV-1 long terminal repeat (LTR) by signal amplification in live cells...

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University of Melbourne Researchers

Christina Cortez-Jugo Author

Roop Bhangu Author

Sharon Lewin Author

Francesca Cavalieri Author

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Grants

Awarded by Horizon 2020 Framework Programme

Funding Acknowledgements

This research was conducted and funded by the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (CE140100036) and the National Health and Medical Research Council (NHMRC) (GNT1149990). F. Caruso acknowledges the award of an NHMRC Senior Principal Research Fellowship (GNT1135806). F. Cavalieri acknowledges the award of an RMIT Senior Vice Chancellor Fellowship. This work received funding from the European Union's Horizon 2020 research and innovation program under the Marie Skodowska-Curie grant agreement no. 690901 ("NANOSUPREMI"). A. Amodio acknowledges funding from the European Union's Horizon 2020 research and innovation program under the Marie Skodowska-Curie grant agreement no. 798565 ("RE-IMMUNE"). G. Forte acknowledges the European Regional Development Fund - project MAGNET (no. CZ.02.1.01/0.0/0.0/15_003/0000492) and the European Regional Development Fund - project ENOCH (no. CZ.02.1.01/0.0/0.0/16_019/0000868). This work was performed in part at the Materials Characterization and Fabrication Platform (MCFP) at The University of Melbourne.