Journal article
Structure of the Pf12 and Pf41 heterodimeric complex of Plasmodium falciparum 6-cysteine proteins
MH Dietrich, LJ Chan, A Adair, C Boulet, MT O'Neill, LL Tan, S Keremane, YF Mok, AW Lo, P Gilson, WH Tham
FEMS Microbes | Published : 2022
Abstract
During the different stages of the Plasmodium life cycle, surface-Associated proteins establish key interactions with the host and play critical roles in parasite survival. The 6-cysteine (6-cys) protein family is one of the most abundant surface antigens and expressed throughout the Plasmodium falciparum life cycle. This protein family is conserved across Plasmodium species and plays critical roles in parasite transmission, evasion of the host immune response and host cell invasion. Several 6-cys proteins are present on the parasite surface as hetero-complexes but it is not known how two 6-cys proteins interact together. Here, we present a crystal structure of Pf12 bound to Pf41 at 2.85 Å r..
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Awarded by Wellcome Trust
Funding Acknowledgements
We acknowledge the traditional custodians of the lands on which this project was conducted, the Wurundjeri and the Bunurong people of the Kulin Nation. We thank Janet Newman and Bevan Marshall from the CSIRO Collaborative Crystallization Centre (www.csiro.au/C3) (CSIRO; Parkville, Australia) for assistance with setting up the crystallization screens. This research was undertaken using the MX2 beamline at the Australian Synchrotron, part of ANSTO, and made use of the Australian Cancer Research Foundation (ACRF) detector. We also thank the MX2 beamline staff at the Australian Synchrotron for their assistance during data collection. We thank Michael Lawrence for his invaluable advice on the structure refinement of the heterodimeric complex of Pf12 and Pf41. W.-H.T. is a Howard Hughes Medical Institute-Wellcome Trust International Research Scholar (208693/Z/17/Z) and supported by National Health and Medical Research Council of Australia (APP2001385, GNT1143187, GNT1160042, GNT1160042, GNT1154937). We thank The Australian Red Cross Blood Bank for the supply of human erythrocytes.