Journal article
CXCL11 expressing C57BL/6 mice have intact adaptive immune responses to viral infection
L Dalit, C Alvarado, L Küijper, AJ Kueh, A Weir, A D’Amico, MJ Herold, JE Vince, SL Nutt, JR Groom
Immunology and Cell Biology | Published : 2022
DOI: 10.1111/imcb.12541
Open access
Abstract
The chemokine receptor CXCR3 is expressed on immune cells to co-ordinate lymphocyte activation and migration. CXCR3 binds three chemokine ligands, CXCL9, CXCL10 and CXCL11. These ligands display distinct expression patterns and ligand signaling biases; however, how each ligand functions individually and collaboratively is incompletely understood. CXCL9 and CXCL10 are considered pro-inflammatory chemokines during viral infection, while CXCL11 may induce a tolerizing state. The investigation of the individual role of CXCL11 in vivo has been hampered as C57BL/6 mice carry several mutations that result in a null allele. Here, CRISPR/Cas9 was used to correct these mutations on a C57BL/6 backgroun..
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Awarded by Australian Government
Funding Acknowledgements
We acknowledge the late Dr Amanda Proudfoot for discussions at the conception of this study and Dr Nicolas Jacquelot for technical help and discussions. The CXCR3 ligand scheme was generated using Biorender. CXCL11KI mice were generated by the WEHI MAGEC laboratory supported by Phenomics Australia and the Australian Government through the National Collaborative Research Infrastructure Strategy (NCRIS) program. This work was supported by National Health and Medical Research Council (NHMRC) Investigator grant (2007812), Ideas grant (1182649) and Project grant (1137989) grants to JRG. LD is supported by Melbourne research scholarships. JRG was supported by Australian Research Council Future Fellowship (FT130100708) and WEHI Centenary Fellowship sponsored by CSL. JEV was supported by an NHMRC Ideas grant (1183070) and fellowship (1141466). MJH is supported by the Leukemia and Lymphoma Society of America (LLS SCOR 7015-18) and NHMRC Ideas Grant (118657). This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. Open access publishing facilitated by The University of Melbourne, as part of the Wiley -The University of Melbourne agreement via the Council of Australian University Librarians.