Differential location of NKT and MAIT cells within lymphoid tissue

DN Johnson; Z Ruan; EV Petley; S Devi; LE Holz; AP Uldrich; JYW Mak; JL Hor; SN Mueller; J McCluskey; DP Fairlie; PK Darcy; PA Beavis; WR Heath; DI Godfrey

Journal article

Differential location of NKT and MAIT cells within lymphoid tissue

DN Johnson, Z Ruan, EV Petley, S Devi, LE Holz, AP Uldrich, JYW Mak, JL Hor, SN Mueller, J McCluskey, DP Fairlie, PK Darcy, PA Beavis, WR Heath, DI Godfrey

Scientific Reports | Published : 2022

DOI: 10.1038/s41598-022-07704-4

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Abstract

Natural Killer T (NKT) cells and Mucosal-Associated Invariant T (MAIT) cells are innate-like T cells that express semi-invariant αβ T cell receptors (TCRs) through which they recognise CD1d and MR1 molecules, respectively, in complex with specific ligands. These cells play important roles in health and disease in many organs, but their precise intra-organ location is not well established. Here, using CD1d and MR1 tetramer staining techniques, we describe the precise location of NKT and MAIT cells in lymphoid and peripheral organs. Within the thymus, NKT cells were concentrated in the medullary side of the corticomedullary junction. In spleen and lymph nodes, NKT cells were mainly localised w..

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University of Melbourne Researchers

Darryl Johnson Author

Zheng Ruan Author

Emma Petley Author

Sapna Devi Author

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Awarded by National Institute of Allergy and Infectious Diseases

Funding Acknowledgements

The authors wish to thank Graham Anderson, Sidonia Eckle and Lyudmila Kostenko for intellectual input and technical assistance, Ellie Cho for assistance in bioimage analysis, Paul Savage for providing PBS-44, and Olivier Lantz for kindly supplying B6-MAITCAST mice. All image acquisition was performed on microscopes maintained by the Biological Optical Microscopy Platform (BOMP), The University of Melbourne. We acknowledge the support of an Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging (Grant CE140100011) and the National Health and Medical Research Council (NHMRC) program Grant (1113293); project Grant (APP1125493); and research fellowships (APP1117017; 1117766, 1136680).