Journal article
Fibrin clot characteristics and anticoagulant response in a SARS-CoV-2-infected endothelial model
Conor McCafferty, Leo Lee, Tengyi Cai, Slavica Praporski, Julian Stolper, Vasiliki Karlaftis, Chantal Attard, David Myint, Leeanne M Carey, David W Howells, Geoffrey A Donnan, Stephen Davis, Henry Ma, Sheila Crewther, Vinh A Nguyen, Suelyn Van Den Helm, Natasha Letunica, Ella Swaney, David Elliott, Kanta Subbarao Show all
EJHAEM | WILEY | Published : 2022
DOI: 10.1002/jha2.407
Abstract
Coronavirus disease 2019 (COVID-19) patients have increased thrombosis risk. With increasing age, there is an increase in COVID-19 severity. Additionally, adults with a history of vasculopathy have the highest thrombotic risk in COVID-19. The mechanisms of these clinical differences in risk remain unclear. Human umbilical vein endothelial cells (HUVECs) were infected with SARS-CoV-2, influenza A/Singapore/6/86 (H1N1) or mock-infected prior to incubation with plasma from healthy children, healthy adults or vasculopathic adults. Fibrin on surface of cells was observed using scanning electron microscopy, and fibrin characteristics were quantified. This experiment was repeated in the presence of..
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