Journal article
Diffusion and distal linkages govern interchromosomal dynamics during meiotic prophase
TAC Newman, B Beltran, JM McGehee, D Elnatan, CK Cahoon, MR Paddy, DB Chu, AJ Spakowitz, SM Burgess
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2022
Open access
Abstract
The pairing of homologous chromosomes (homologs) in meiosis is essential for distributing the correct numbers of chromosomes into haploid gametes. In budding yeast, pairing depends on the formation of 150 to 200 Spo11-mediated double-strand breaks (DSBs) that are distributed among 16 homolog pairs, but it is not known if all, or only a subset, of these DSBs contribute to the close juxtaposition of homologs. Having established a system to measure the position of fluorescently tagged chromosomal loci in three-dimensional space over time, we analyzed locus trajectories to determine how frequently and how long loci spend colocalized or apart. Continuous imaging revealed highly heterogeneous cell..
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Awarded by University of California, Davis
Funding Acknowledgements
We thank the laboratory of Angelika Amon for our FROS strains, and we appreciate discussions with and feedback from Amy MacQueen. We also thank the Light Microscopy Imaging Facility (Molecular and Cellular Biology, University of California, Davis) . This work was supported by NIH Grant R01 GM075119 to S.M.B. B.B. acknowledges funding support from NSF Graduate Fellowship Program DGE-1656518 and from NIH Training Grant T32GM008294. Financial support for A.J.S. is provided by NSF Physics of Living Systems Program Grant PHY-1707751.