Journal article
Observations of phase changes in monoolein during high viscous injection
DJ Wells, P Berntsen, E Balaur, CM Kewish, P Adams, A Aquila, J Binns, S Boutet, H Broomhall, C Caleman, A Christofferson, CE Conn, C Dahlqvist, L Flueckiger, FG Roque, TL Greaves, M Hejazian, M Hunter, MH Jazi, HO Jönsson Show all
Journal of Synchrotron Radiation | INT UNION CRYSTALLOGRAPHY | Published : 2022
Abstract
Serial crystallography of membrane proteins often employs high-viscosity injectors (HVIs) to deliver micrometre-sized crystals to the X-ray beam. Typically, the carrier medium is a lipidic cubic phase (LCP) media, which can also be used to nucleate and grow the crystals. However, despite the fact that the LCP is widely used with HVIs, the potential impact of the injection process on the LCP structure has not been reported and hence is not yet well understood. The self-assembled structure of the LCP can be affected by pressure, dehydration and temperature changes, all of which occur during continuous flow injection. These changes to the LCP structure may in turn impact the results of X-ray di..
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Awarded by National Science Foundation
Funding Acknowledgements
The following funding is acknowledged: Australian Research Council (grant No. DP190103027 awarded to AVM, CD, TLG, PB); BioXFEL Science and Technology Center (award No. DBI-1231306 to RK); ARC Centre of Excellenc in Advance Molecular Imaging (grant No. CE140100011); ANSTO International Synchrotron Acess Program (ISAP) (award No. AS/IA152/10143 to CD). National Science Foundation awards (1231306, 1943448 and 1817862 to RAK), Swedish Research Council (Grant 2018-00740 to CC). Part of the sample injector used at LCLS for this research was funded by the National Institutes of Health (P41GM103393, formerly P41RR001209 to SB). Use of the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, is supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences (contract No. DE-AC02-76SF00515).