Journal article
Template-Assisted Antibody Assembly: A Versatile Approach for Engineering Functional Antibody Nanoparticles
Y Hu, J Li, Y Ju, ZH Houston, NL Fletcher, R De Rose, S Fernandes, CE Hagemeyer, K Alt, KJ Thurecht, C Cortez-Jugo, F Caruso
Chemistry of Materials | American Chemical Society | Published : 2022
Abstract
The clinical success of monoclonal antibody therapy has inspired research in understanding the fundamental molecular basis of antibody-antigen interactions and the engineering of antibodies and antibody assemblies with enhanced or novel properties. In particular, colloidally stable antibody assemblies can enhance dosing strategies and enable combined therapy of a mixture of antibodies or biologics. Herein, nanoassemblies of therapeutic antibodies were fabricated with controlled physicochemical properties using a versatile template-mediated assembly method. The antibody nanoparticles (AbNPs) cross-linked with poly(ethylene glycol)-N-hydroxysuccinimide were monodispersed, with particle diamete..
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Grants
Awarded by Horizon 2020 Framework Programme
Funding Acknowledgements
This research was conducted and funded by the Australian Research Council Centre of Excellence in Convergent BioNano Science and Technology (project number CE140100036). C.E.H. and F.C. acknowledge the award of a National Health and Medical Research Council (NHMRC) Project Grant (GNT1138361) that also supported this work. F.C. acknowledges the award of an NHMRC Senior Principal Research Fellowship (GNT1135806). This work received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 690901 ("NANOSUPREMI"). K.A. acknowledges the award of an NHMRC Career Development Fellowship (GNT1140465). This work was performed in part at the Materials Characterisation and Fabrication Platform (MCFP) at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility (ANFF). We thank Dr Andrew Mitchell (MCFP) for his valuable contribution in obtaining ethics approval.