Journal article
CD90 Marks a Mesenchymal Program in Human Thymic Epithelial Cells In Vitro and In Vivo
S Sun, JY Li, HT Nim, A Piers, M Ramialison, ER Porrello, IE Konstantinov, AG Elefanty, EG Stanley
Frontiers in Immunology | Published : 2022
Open access
Abstract
Thymic epithelium is critical for the structural integrity of the thymus and for T cell development. Within the fully formed thymus, large numbers of hematopoietic cells shape the thymic epithelium into a scaffold-like structure which bears little similarity to classical epithelial layers, such as those observed in the skin, intestine or pancreas. Here, we show that human thymic epithelial cells (TECs) possess an epithelial identity that also incorporates the expression of mesenchymal cell associated genes, whose expression levels vary between medullary and cortical TECs (m/cTECs). Using pluripotent stem cell (PSC) differentiation systems, we identified a unique population of cells that co-e..
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Awarded by Murdoch Children's Research Institute
Funding Acknowledgements
This study was funded by the National Health & Medical Research Council of Australia through research fellowships awarded to AE (GNT1117596) and ES (GNT1079004) and project grants awarded to AE and ES (GNT1129861, GNT1138717, GNT1123277), and by the Stafford Fox Medical Research Foundation. MR is funded by an NHMRC Ideas Grant (APP1180905). Additional infrastructure funding to the Murdoch Childrens Research Institute was provided by the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme and the Victorian Governments Operational Infrastructure Support Program. The Novo Nordisk Foundation Center for Stem Cell Medicine is supported by Novo Nordisk Foundation grants (NNF21CC0073729). The Melbourne Centre for Cardiovascular Genomics and Regenerative Medicine (CardioRegen) and the Melbourne Childrens Heart Tissue Bank (MCHTB) are funded by the RCH Foundation, Shine On Foundation and the Loti and Victor Smorgon Family Foundation.