Expression of monoclonal antibodies for functional and structural studies

Abstract

Monoclonal antibodies are a mainstay of the research laboratory, with increasing focus on human IgG that best reflects the human antibody response and allows swift transition to therapeutic monoclonal development. The technologies to express human IgG in the laboratory are increasingly accessible, allowing researchers in diverse fields to produce human monoclonals for functional and structural studies. In this chapter, we provide an overview of the considerations for expressing recombinant IgG, with reference to detailed protocols and reputed sources for reagents. These recombinant antibodies can then be evaluated in functional assays, specific to their antigen targets and disease state. We ..