Journal article
Distinctive molecular features of regenerative stem cells in the damaged male germline
HM La, J Liao, JMD Legrand, FJ Rossello, AL Chan, V Vaghjiani, JE Cain, A Papa, TL Lee, RM Hobbs
Nature Communications | Published : 2022
Open access
Abstract
Maintenance of male fertility requires spermatogonial stem cells (SSCs) that self-renew and generate differentiating germ cells for production of spermatozoa. Germline cells are sensitive to genotoxic drugs and patients receiving chemotherapy can become infertile. SSCs surviving treatment mediate germline recovery but pathways driving SSC regenerative responses remain poorly understood. Using models of chemotherapy-induced germline damage and recovery, here we identify unique molecular features of regenerative SSCs and characterise changes in composition of the undifferentiated spermatogonial pool during germline recovery by single-cell analysis. Increased mitotic activity of SSCs mediating ..
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Awarded by Victorian Cancer Agency
Funding Acknowledgements
We thank the Monash Animal Research Platform, Micromon, Monash FlowCore, Monash Bioinformatics Platform, Monash Micro Imaging and Monash Health Translation Precinct Medical (MHTP) Genomics, Microscopy, Histology and Flow Cytometry Platforms for technical support. This study was supported by NHMRC Project Grant APP1164019 to R.M.H. H.M.L. was supported by an Australian Government Research Training Program (RTP) Scholarship and A.P. by a Victorian Cancer Agency (VCA) Mid-Career Research Fellowship (MCRF20027). The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and Australian Government.