Journal article
Elevation of fatty acid desaturase 2 in esophageal adenocarcinoma increases polyunsaturated lipids and may exacerbate bile acid-induced DNA damage
Jeffrey Molendijk, Cathryn M Kolka, Henry Cairns, Sandra Brosda, Ahmed Mohamed, Alok K Shah, Ian Brown, Mark P Hodson, Thomas Hennessy, Guanghao Liu, Thomas Stoll, Renee S Richards, Michael Gartside, Kalpana Patel, Nicholas J Clemons, Wayne A Phillips, Andrew Barbour, Johan A Westerhuis, Michelle M Hill
CLINICAL AND TRANSLATIONAL MEDICINE | JOHN WILEY & SONS LTD | Published : 2022
DOI: 10.1002/ctm2.810
Abstract
BACKGROUND: The risk of esophageal adenocarcinoma (EAC) is associated with gastro-esophageal reflux disease (GERD) and obesity. Lipid metabolism-targeted therapies decrease the risk of progressing from Barrett's esophagus (BE) to EAC, but the precise lipid metabolic changes and their roles in genotoxicity during EAC development are yet to be established. METHODS: Esophageal biopsies from the normal epithelium (NE), BE, and EAC, were analyzed using concurrent lipidomics and proteomics (n = 30) followed by orthogonal validation on independent samples using RNAseq transcriptomics (n = 22) and immunohistochemistry (IHC, n = 80). The EAC cell line FLO-1 was treated with FADS2 selective inhibitor ..
View full abstractGrants
Awarded by National Health and Medical Research Council (NHMRC) of Australia
Awarded by Cancer Council NSW
Funding Acknowledgements
Translational Research Institute Spore Grant, University of Queensland International Postgraduate Research Scholarship. PROBE-NET was funded by the National Health and Medical Research Council (NHMRC) of Australia (NHMRC APP1040947) and Cancer Council NSW (SRP 08-04).