The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis

EA Maunders; DHY Ngu; K Ganio; SI Hossain; BYJ Lim; MG Leeming; Z Luo; A Tan; E Deplazes; B Kobe; CA McDevitt

Journal article

The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis

EA Maunders, DHY Ngu, K Ganio, SI Hossain, BYJ Lim, MG Leeming, Z Luo, A Tan, E Deplazes, B Kobe, CA McDevitt

Frontiers in Microbiology | FRONTIERS MEDIA SA | Published : 2022

DOI: 10.3389/fmicb.2022.903146

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Abstract

Acquisition of the trace-element molybdenum via the high-affinity ATP-binding cassette permease ModABC is essential for Pseudomonas aeruginosa respiration in anaerobic and microaerophilic environments. This study determined the X-ray crystal structures of the molybdenum-recruiting solute-binding protein ModA from P. aeruginosa PAO1 in the metal-free state and bound to the group 6 metal oxyanions molybdate, tungstate, and chromate. Pseudomonas aeruginosa PAO1 ModA has a non-contiguous dual-hinged bilobal structure with a single metal-binding site positioned between the two domains. Metal binding results in a 22° relative rotation of the two lobes with the oxyanions coordinated by four residue..

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University of Melbourne Researchers

Bryan Lim Author

Michael Leeming Author

Aimee Tan Author

Christopher McDevitt Author

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Grants

Awarded by University of Queensland

Funding Acknowledgements

This work was supported by the National Health and Medical Research Council (NHMRC) grants 1071659 to BK, 1122582 to CM and 1180826 to BK and CM. BK is an ARC Laureate Fellow (FL180100109) and CM is an ARC Future Fellow (FT170100006).