Journal article
Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems
S Ahmerkamp, FM Jalaluddin, Y Cui, DR Brumley, CO Pacherres, JS Berg, R Stocker, MMM Kuypers, K Koren, L Behrendt
Cell Reports Methods | Published : 2022
Abstract
From individual cells to whole organisms, O2 transport unfolds across micrometer- to millimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O2 dynamics via currently available methods difficult or unreliable. Here, we present “sensPIV,” a method to simultaneously measure O2 concentrations and flow fields. By tracking O2-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, we measured O2 transport within (1) microfluidic devices, (2) sinking model aggregates, and (3) complex colony-forming corals. Through th..
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Awarded by Simons Foundation
Funding Acknowledgements
We thank Volker Meyer, Paul Faerber, and Gerhard Holst for technical support. We thank Claudio Richter for providing the coral Porites lutea. We thank Sina Schorn, Bram Vekeman, and Juliane Schoetz for support in the laboratory. We thank Dirk de Beer for valuable discussions and comments. D.R.B. was supported by an Australian Research Council (ARC) Discovery Early Career Researcher Award DE180100911 and a Gordon & Betty Moore Foundation Symbiosis in Aquatic Systems grant. C.O.P. acknowledges funding from the Helmholtz Association (Alfred Wegener Institute, Helmholtz Center for Polar and Marine Research). F.M.J. received funding from the Cluster of Excellence "The Ocean Floor-Earth's Uncharted Interface" (Germany's Excellence Strategy-EXC-2077-390741603 of the DFG). L.B. and Y.C. were supported by grants from the Swedish Research Council (2019-04401) and the Science for Life Laboratory. R.S. acknowledges funding from the Simons Foundation through the Principles of Microbial Ecosystems (PriME) Collaborative (grant 542395). RS acknowledges support from a Symbiosis in Aquatic Systems Investigator Award from the Gordon and Betty Moore Foundation (GBMF9197; https://doi.org/10.37807/GBMF9197). K.K. acknowledges financial support by research grants from the Grundfos Foundation and a Sapere Aude grant from the Independent Research Fund Denmark (IRFD): DFF-8048-00057B. The study was funded by the Max Planck Society (MPG) through the "Multiscale Approach on the Role of Marine Aggregates" (MARMA) project.