Journal article
Protein precoating modulates biomolecular coronas and nanocapsule-immune cell interactions in human blood
S Li, Y Ju, J Zhou, M Faria, CS Ang, AJ Mitchell, QZ Zhong, T Zheng, SJ Kent, F Caruso
Journal of Materials Chemistry B | Published : 2022
DOI: 10.1039/d2tb00672c
Abstract
The biomolecular corona that forms on particles upon contact with blood plays a key role in the fate and utility of nanomedicines. Recent studies have shown that precoating nanoparticles with serum proteins can improve the biocompatibility and stealth properties of nanoparticles. However, it is not fully clear how precoating influences biomolecular corona formation and downstream biological responses. Herein, we systematically examine three precoating strategies by coating bovine serum albumin (single protein), fetal bovine serum (FBS, mixed proteins without immunoglobulins), or bovine serum (mixed proteins) on three nanoparticle systems, namely supramolecular template nanoparticles, metal-p..
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Grants
Awarded by Australian National Fabrication Facility
Funding Acknowledgements
This research was supported by the Australian Research Council Discovery Project (DP210103114). F. C. acknowledges the award of a National Health and Medical Research Council Senior Principal Research Fellowship (GNT1135806). Y. J. acknowledges the award of an Early Career Researcher Grant from The University of Melbourne (ECR1032020) and an RMIT Vice-Chancellor's Postdoctoral Fellowship from RMIT University. This work was performed in part at the Materials Characterisation and Fabrication Platform at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility. The authors acknowledge Dr Hannah Gabrielle Kelly for helpful discussions and assistance with the whole blood assays.