Journal article
Multiparametric High-Content Cell Painting Identifies Copper Ionophores as Selective Modulators of Esophageal Cancer Phenotypes
RE Hughes, RJR Elliott, X Li, AF Munro, A Makda, RN Carter, NM Morton, K Fujihara, NJ Clemons, R Fitzgerald, JR O'Neill, T Hupp, NO Carragher
ACS Chemical Biology | Published : 2022
Abstract
Esophageal adenocarcinoma is of increasing global concern due to increasing incidence, a lack of effective treatments, and poor prognosis. Therapeutic target discovery and clinical trials have been hindered by the heterogeneity of the disease, the lack of "druggable" driver mutations, and the dominance of large-scale genomic rearrangements. We have previously undertaken a comprehensive small-molecule phenotypic screen using the high-content Cell Painting assay to quantify the morphological response to a total of 19,555 small molecules across a panel of genetically distinct human esophageal cell lines to identify new therapeutic targets and small molecules for the treatment of esophageal aden..
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Awarded by Victorian Cancer Agency
Funding Acknowledgements
This study was supported by an MRC Institute of Genetics and Molecular Medicine Ph.D. studentship Award to R.E.H., the Anne Forrest Fund for Esophageal Cancer Research, and a CRUK Small-Molecule Drug Discovery Project Award to N.O.C. (C42454/A24892) . R.N.C. and N.M.M. were supported by a Wellcome Trust New Investigator Award (100981/Z/13/Z) to N.M.M. N.J.C. was awarded a National Health and Medical Research Council (NHMRC) Project Grant (APP1120293) and is supported by a Fellowship (MCRF16002) from the Department of Health and Human Services acting through the Victorian Cancer Agency. K.F. is supported by an Australian Research Training Program (RTP) Scholarship. The laboratory of RCF is funded by a Programme Grant from the Medical Research Council (MR/W014122/1, G111260) .