Journal article
High-throughput microfluidic real-time PCR for the simultaneous detection of selected vector-borne pathogens in dogs in Bosnia and Herzegovina
V Colella, L Huggins, A Hodžić, C Galon, R Traub, A Alić, R Iatta, L Halos, D Otranto, M Vayssier-Taussat, S Moutailler
Transboundary and Emerging Diseases | WILEY-HINDAWI | Published : 2022
DOI: 10.1111/tbed.14645
Abstract
A scarcity of information on the occurrence of zoonotic vector-borne pathogens (VBPs), alongside a lack of human and animal health authorities’ awareness of pre-existing data, augment the risk of VBP infection for local people and limit our ability to establish control programs. This holds especially true in low-middle income countries such as Bosnia and Herzegovina (BiH). This dearth of information on zoonotic VBPs is bolstered by the inability of previously used diagnostic tests, including conventional molecular diagnostic methods, to detect the full spectrum of relevant pathogens. Considering this, we set out to apply a microfluidic qPCR assay capable of detecting 43 bacterial and protozo..
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Awarded by Agence Nationale de la Recherche
Funding Acknowledgements
CG, MV and SM research is supported by the French Agency for Food, Environmental and Occupational Health and Safety (ANSES), the French National Institute for Agricultural Research (INRAE), and the National Agency of Research (ANR OhTicks!). UMR BIPAR is supported by the French Government's Investissement d'Avenir program, Laboratoire d'Excellence "Integrative Biology of Emerging Infectious Diseases" (grant No. ANR-10-LABEX-62-IBEID). Authors thank The European Network for Neglected Vectors & Vector-Borne Infections (EurNegVec TD1303), for supporting the collection of samples used in this study. Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.