18 F Site‐Specific Labelling of a Single‐Chain Antibody against Activated Platelets for the Detection of Acute Thrombosis in Positron Emission Tomography

Abstract

Positron emission tomography is the imaging modality of choice when it comes to the high sensitivity detection of key markers of thrombosis and inflammation, such as activated platelets. We, previously, generated a fluorine‐18 labelled single‐chain antibody (scFv) against ligand‐induced binding sites (LIBS) on activated platelets, binding it to the highly abundant platelet glycoprotein integrin receptor IIb/IIIa. We used a non‐site‐specific bio conjugation approach with N‐succinimidyl‐ 4‐[18F]fluorobenzoate (S[18F]FB), leading to a mixture of products with reduced antigen binding. In the present study, we have developed and characterised a novel fluorine‐18 PET radiotracer, based on this ant..