Characterizing Conformational Diversity of G Protein-coupled Receptors by Solution NMR Spectroscopy

Abstract

G protein-coupled receptors are the largest family of integral membrane proteins in humans that have roles in almost all physiological processes. The binding of extracellular ligands allosterically modulates the intracellular interaction of the GPCR with transducer proteins such as G proteins and arrestins. This allosteric coupling operates via a network of conserved microswitches to adjust the equilibrium of active, intermediate and inactive states of the GPCR. Crystallography and cryo-electron microscopy have determined the structures of many active and inactive state GPCRs, while solution-state methods such as NMR spectroscopy inform on the dynamics of additional states and their role in ..