Journal article
Direct Assessment of Rabbit Cortical Bone Basic Multicellular Unit Longitudinal Erosion Rate: A 4D Synchrotron-Based Approach
KD Harrison, E Sales, BD Hiebert, A Panahifar, N Zhu, T Arnason, KJ Swekla, P Pivonka, LD Chapman, DML Cooper
Journal of Bone and Mineral Research | OXFORD UNIV PRESS | Published : 2022
DOI: 10.1002/jbmr.4700
Open access
Abstract
Cortical bone remodeling is carried out by basic multicellular units (BMUs), which couple resorption to formation. Although fluorochrome labeling has facilitated study of BMU formative parameters since the 1960s, some resorptive parameters, including the longitudinal erosion rate (LER), have remained beyond reach of direct measurement. Indeed, our only insights into this spatiotemporal parameter of BMU behavior come from classical studies that indirectly inferred LER. Here, we demonstrate a 4D in vivo method to directly measure LER through in-line phase contrast synchrotron imaging. The tibias of rabbits (n = 15) dosed daily with parathyroid hormone were first imaged in vivo (synchrotron mic..
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Awarded by University of Saskatchewan
Funding Acknowledgements
Part or all of the research described in this article was performed at the Canadian Light Source, a national research facility of the University of Saskatchewan, which is supported by the Canada Foundation for Innovation (CFI), the Natural Sciences and Engineering Research Council (NSERC), the National Research Council (NRC), the Canadian Institutes of Health Research (CIHR), the Government of Saskatchewan, and the University of Saskatchewan. This specific research was primarily supported by a CIHR Catalyst Grant (FRN: 151724). We also acknowledge Dr. George Belev's significant contributions toward the implementation of the in vivo imaging portion of this research, as well as Gavin King for his assistance with production of images for the article. We also thank Dr. Ingrid Pickering and Dr. Olena Ponomarenko for their contributions of resources and time regarding the confocal histological analyses portion of this research. We also acknowledge Dr. Denver Marchiori for his assistance with the data processing for the revision of this article. Finally, the authors acknowledge the support of the University of Saskatchewan Laboratory Animals Service Unit (LASU), which was essential for the success of this research. Authors' roles: KDH: conceptualization, data curation, formal analysis, investigation, methodology, project administration, visualization, writing-original draft, and writing-review & editing. ES: formal analysis, methodology, visualization, writing-original draft, and writing-review & editing. BDH: data curation, methodology, and writing-review & editing. AP: conceptualization, data curation, funding acquisition, investigation, methodology, visualization, writing-original draft, and writing-review & editing. NZ: data curation and writing-review & editing. TA: conceptualization, funding acquisition, methodology, and writing-review & editing. KJS: data curation, investigation, methodology, and writing-review & editing. PP: conceptualization, funding acquisition, methodology, writing-original draft, and writing-review & editing. DC: conceptualization, methodology, and writing-review & editing. DMLC: conceptualization, data curation, formal analysis, funding acquisition, investigation, methodology, project administration, supervision, validation, visualization, writing-original draft, and writing-review & editing.