Proteome-wide systems genetics identifies UFMylation as a regulator of skeletal muscle function

J Molendijk; R Blazev; RJ Mills; YK Ng; KI Watt; D Chau; P Gregorevic; PJ Crouch; JBW Hilton; L Lisowski; P Zhang; K Reue; AJ Lusis; JE Hudson; DE James; MM Seldin; BL Parker

Journal article

Proteome-wide systems genetics identifies UFMylation as a regulator of skeletal muscle function

J Molendijk, R Blazev, RJ Mills, YK Ng, KI Watt, D Chau, P Gregorevic, PJ Crouch, JBW Hilton, L Lisowski, P Zhang, K Reue, AJ Lusis, JE Hudson, DE James, MM Seldin, BL Parker

Elife | eLIFE SCIENCES PUBL LTD | Published : 2022

DOI: 10.7554/ELIFE.82951

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Abstract

Improving muscle function has great potential to improve the quality of life. To identify novel regulators of skeletal muscle metabolism and function, we performed a proteomic analysis of gastrocnemius muscle from 73 genetically distinct inbred mouse strains, and integrated the data with previously acquired genomics and >300 molecular/phenotypic traits via quantitative trait loci mapping and correlation network analysis. These data identified thousands of associations between protein abundance and phenotypes and can be accessed online (https://muscle.coffeeprot.com/) to identify regulators of muscle function. We used this resource to prioritize targets for a functional genomic screen in huma..

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University of Melbourne Researchers

Ronnie Blazev Author

Richard Mills Author

Kevin Watt Author

Paul Gregorevic Author

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Awarded by University of Sydney

Funding Acknowledgements

National Health and Medical Research Council APP1184363 Karen Reue Marcus M Seldin Benjamin L ParkerNational Health and Medical Research Council APP2009642 Benjamin L ParkerNational Health and Medical Research Council APP2013189 Richard J MillsNational Health and Medical Research Council APP1156562 Paul Gregorevic Benjamin L ParkerNational Institutes of Health HL138193 Marcus M SeldinNational Institutes of Health DK130640 Marcus M SeldinNational Institutes of Health DK097771 Marcus M SeldinNational Institutes of Health GM115318 Karen ReueNational Institutes of Health AG070959 Aldons J LusisNational Institutes of Health HL147883 Aldons J LusisNational Institutes of Health DK117850 Aldons J LusisWeary Dunlop Foundation Benjamin L ParkerThe ALS Association 21-DDC-574 Paul Gregorevic Peter J Crouch