Journal article
Helicobacter pylori Growth Stage Determines the Size, Protein Composition, and Preferential Cargo Packaging of Outer Membrane Vesicles
L Zavan, NJ Bitto, EL Johnston, DW Greening, M Kaparakis-Liaskos
Proteomics | Published : 2019
Abstract
Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. However, very little is known regarding the effect of bacterial growth stage on the size, content, and selective packaging of proteins into OMVs. In this study, the global proteome of Helicobacter pylori and their OMVs throughout bacterial growth are examined to determine if bacterial growth stage affected OMV cargo composition. Analysis of OMVs produced by H. pylori reveals that ..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
D. W. G. andM. K-L. contributed equally to this work. This project was supported by a La Trobe University RFA Understanding Diseases Grant (M.K-L), and in part, by the La Trobe University Stone Fellowship (D. W. G), the Australian National Health and Medical Research Council (Project: M. K-L 1107800, D.W.G 1139489,1141964), and funding support from La Trobe University (M. K-L, D.W.G). This work was supported by the LIMS BioImaging Facility (La Trobe University), and the Comprehensive Proteomics Platform (La Trobe University). M. K-L. is a veski Inspiring Women Fellow. L.Z is the recipient of an Australian Society for Microbiology Summer Student Research Award.