Journal article
Protocol for production and expression of chimeric bovine-human monoclonal antibodies
B Heydarchi, DFJ Purcell
STAR Protocols | ELSEVIER | Published : 2022
Abstract
We describe herein a protocol for production of chimeric bovine-human monoclonal antibodies (mAbs) from vaccinated cows. The genes of HIV-1-specific single B cells are amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned into human expression vectors, and expressed in human cell lines. This protocol provides an efficient step-by-step methodology to produce HIV-1 chimeric mAbs and could be widely adapted for other antigens. For complete details on the use and execution of this protocol, please refer to Heydarchi et al. (2022).1
Grants
Awarded by Horizon 2020 Framework Programme
Funding Acknowledgements
This work was conducted under animal ethics approval 2015-17 from the Victorian State Govern-ment DEDJTR Research and Extension Animal Ethics Committee. Funding support was provided to D.F.J.P. from the Australian NHRMC-EU Horizons 2020 Grant (APP1115828) , as a partner in the European AIDS Vaccine Initiative 2020 (EAVI 2020) , from the Australian Centre for HIV-1 and Hepatitis Virology Research, and to B.H. from the Melbourne HIV-1 Cure Consortium and the Australian Centre for HIV-1 and Hepatitis Virology Research.This project has received funding from the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No 681137.