Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon

AJL Leembruggen; Y Lu; H Wang; V Uzungil; T Renoir; AJ Hannan; LA Stamp; MM Hao; JC Bornstein

Journal article

Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon

AJL Leembruggen, Y Lu, H Wang, V Uzungil, T Renoir, AJ Hannan, LA Stamp, MM Hao, JC Bornstein

Biomolecules | Published : 2023

DOI: 10.3390/biom13010139

Download PDF

Abstract

Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is evidence that Group-I metabotropic glutamate receptors (mGlu1 and mGlu5) have established roles in excitatory neurotransmission and synaptic plasticity. While glutamate is abundantly present in the gut, it plays a smaller role in neurotransmission in the enteric nervous system. In this study, we examined the roles of Group-I mGlu receptors in gastrointestinal function. We investigated the expression of Grm1 (mGlu1) and Grm5 (mGlu5) in the mouse myenteric plexus using RNAscope in situ hybridization. Live calcium imaging and motility analysis were performed on ex vivo preparations of the mouse colon...

View full abstract

University of Melbourne Researchers

Anthony Hannan Author

Lincon Stamp Author

Marlene Hao Author

Joel Bornstein Author

Related Projects (3)

Understanding interactions between gut epithelial stem cells and neurons

This project aims to investigate the interaction between gut neurons and the epithelial stem cell compartment, as well as the relationship b..

ROLE OF THE ENTERIC NERVOUS SYSTEM IN ANTIBIOTIC ASSOCIATED DIARRHOEA

This project will identify the role played by the gut's own nervous system in the diarrhoea that is often associated with antibiotic treatme..

Genes underlying enteric neuron subtype differentiation

This project aims to use new RNA-sequencing technology to identify genes important for differentiation of several major enteric neuron subty..

Grants

Awarded by Australian Research Council

Funding Acknowledgements

This study was funded by the NHMRC Australia (GNT 1158952 to J.C.B.). The mGlu5-KO mice were generated using funding from an NHMRC Project Grant to A.J.H. and T.R. (GNT 112688). A.J.L.L. is funded by a Research Training Program scholarship. A.J.H. was supported by an NHMRC Principal Research Fellowship (GNT 1117148). T.R. is a NHMRC Boosting Dementia Research Leadership Fellow (APP1136529); L.A.S. was supported by an ARC DECRA fellowship (DE180100261). M.M.H. is an ARC DECRA fellow (DE190101209).