Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver TRM cells

AM Valencia-Hernandez; T Zillinger; Z Ge; PS Tan; A Cozijnsen; G I. McFadden; MH Lahoud; I Caminschi; W Barchet; WR Heath; D Fernandez-Ruiz

Journal article

Complexing CpG adjuvants with cationic liposomes enhances vaccine-induced formation of liver TRM cells

AM Valencia-Hernandez, T Zillinger, Z Ge, PS Tan, A Cozijnsen, G I. McFadden, MH Lahoud, I Caminschi, W Barchet, WR Heath, D Fernandez-Ruiz

Vaccine | Published : 2023

DOI: 10.1016/j.vaccine.2022.12.047

Abstract

Tissue resident memory T cells (TRM cells) can provide effective tissue surveillance and can respond rapidly to infection. Vaccination strategies aimed at generating TRM cells have shown promise against a range of pathogens. We have previously shown that the choice of adjuvant critically influences CD8+ TRM cell formation in the liver. However, the range of adjuvants tested was limited. Here, we assessed the ability of a broad range of adjuvants stimulating membrane (TLR4), endosomal (TLR3, TLR7 and TLR9) and cytosolic (cGAS, RIG-I) pathogen recognition receptors for their capacity to induce CD8+ TRM formation in a subunit vaccination model. We show that CpG oligodeoxynucleotides (ODN) remai..

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University of Melbourne Researchers

Anton Cozijnsen Author

William Heath Author

Geoffrey McFadden Author

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Grants

Awarded by Deutsches Zentrum für Infektionsforschung

Funding Acknowledgements

We thank Melanie Damtsis and Ming Li for technical assistance, and the members of the W.R.H., L.K.M. and S.M. labs for discussion and the Doherty's animal facility staff for mice husbandry. This work was supported by the National Health and Medical Research Council of Australia; D.F.R (NHMRC 1139486), W.R.H. (NHMRC 1154457, NHMRC 1113293, NHMRC 1124706), I.C. (NHMRC 1124706) . W.B. was supported by the German Center for Infection Research (DZIF: TTU 09.702) and the German Research Foundation (DFG: IRTG 2168).