Journal article
Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol
AM Douek, EI Klein, J Kaslin, PD Currie, AA Ruparelia
Zebrafish | MARY ANN LIEBERT, INC | Published : 2021
Abstract
The Zebrafish Embryo Genotyper (ZEG) device provides a promising tool for genotyping live embryos. Although the gross morphology and survival of embryos after the use of ZEG are unaffected, the cellular and molecular effects of the ZEG protocol remain unknown. To address this, we have examined the integrity of specific tissues, and evaluated the expression of stress-responsive genes to determine the impact of the ZEG protocol. Our analyses reveal that although ZEG results in a low-level acute stress response, no long-lasting effects are evident, supporting its utilization for a variety of downstream assays.
Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This study was supported by the National Health and Medical Research Council of Australia project grants to P.D.C.: APP1139253, APP1136567, and J.K.: GNT1145048, GNT1138870. The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government.