Journal article
Survey of activation-induced genome architecture reveals a novel enhancer of Myc
WF Chan, HD Coughlan, M Ruhle, N Iannarella, C Alvarado, JR Groom, CR Keenan, AJ Kueh, AK Wheatley, GK Smyth, RS Allan, TM Johanson
Immunology and Cell Biology | WILEY | Published : 2023
DOI: 10.1111/imcb.12626
Open access
Abstract
The transcription factor Myc is critically important in driving cell proliferation, a function that is frequently dysregulated in cancer. To avoid this dysregulation Myc is tightly controlled by numerous layers of regulation. One such layer is the use of distal regulatory enhancers to drive Myc expression. Here, using chromosome conformation capture to examine B cells of the immune system in the first hours after their activation, we reveal a previously unidentified enhancer of Myc. The interactivity of this enhancer coincides with a dramatic, but discrete, spike in Myc expression 3 h post-activation. However, genetic deletion of this region, has little impact on Myc expression, Myc protein ..
View full abstractGrants
Awarded by Australian Research Council
Funding Acknowledgements
The authors thank the staff of the core facilities at the Walter and Eliza Hall Institute. The authors also thank J. Tellier for generous provision of reagents and insight. This work was supported by grants and fellowships from the Marian and E.H. Flack Fellowship (HDC), the National Health and Medical Research Council of Australia (CRK #1125436, TMJ #1124081, RSA #1100451, GKS & RSA #1158531) and the Australian Research Council (RSA. #130100541). This study was made possible through Victorian State Government Operational Infrastructure Support, the Australian Government NHMRC Independent Research Institute Infrastructure Support scheme, and the Australian Cancer Research Fund. The generation of Myce-/- mice used in this study was supported by Phenomics Australia and the Australian Government through the National Collaborative Research Infrastructure Strategy program. Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.