Journal article
Detrimental actions of obesity-associated advanced glycation end-products on endometrial epithelial cell proliferation are alleviated by antioxidants
JC Hutchison, J Evans, TA Edgell, G Nie, DK Gardner, LA Salamonsen
Reproductive Biomedicine Online | Published : 2023
Abstract
Research question: Advanced glycation end-products (AGE) are elevated in the uterine environment of obese infertile women. Can the detrimental effects of AGE on endometrial epithelial cells be mitigated with therapeutics, and recapitulated in a more physiologically relevant primary model (organoids)? Design: Human endometrial epithelial cells (ECC-1) were exposed to AGE at concentrations physiologically representative of uterine fluid in lean or obese individuals, and three potential therapeutics: 25 nmol/l receptor for AGE (RAGE) antagonist FPS-ZM1, 100 μmol/l metformin, or a combination of antioxidants (10 μmol/l N-acetyl-L-cysteine, 10 μmol/l N-acetyl-L-carnitine and 5 μmol/l α-lipoic aci..
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Funding Acknowledgements
This research was supported by the Victorian Government's Operational Infrastructure Support Program to the Hudson Institute of Medical Research. J.C. H. was supported by an Australian Government Research Training Program (RTP) Scholarship, and J.E. by a Fielding Foundation Fellowship. D.K.G. is supported by University of Melbourne.