Journal article
Supramolecular Polyphenol-DNA Microparticles for In Vivo Adjuvant and Antigen Co-Delivery and Immune Stimulation
Yijiao Qu, Robert De Rose, Chan-Jin Kim, Jiajing Zhou, Zhixing Lin, Yi Ju, Sukhvir Kaur Bhangu, Christina Cortez-Jugo, Francesca Cavalieri, Frank Caruso
Angewandte Chemie International Edition | Wiley | Published : 2023
Abstract
DNA-based materials have attracted interest due to the tunable structure and encoded biological functionality of nucleic acids. A simple and general approach to synthesize DNA-based materials with fine control over morphology and bioactivity is important to expand their applications. Here, we report the synthesis of DNA-based particles via the supramolecular assembly of tannic acid (TA) and DNA. Uniform particles with different morphologies are obtained using a variety of DNA building blocks. The particles enable the co-delivery of cytosine-guanine adjuvant sequences and the antigen ovalbumin in model cells. Intramuscular injection of the particles in mice induces antigen-specific antibody p..
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Grants
Awarded by Australian National Health and Medical Research Council (NHMRC)
Awarded by NHMRC Senior Principal Research Fellowship
Funding Acknowledgements
This research was funded by the Australian National Health and Medical Research Council (NHMRC; Program Grant GNT1149990). F. Caruso acknowledges the award of an NHMRC Senior Principal Research Fellowship (GNT1135806). Y.Q. acknowledges support from the China Scholarship Council. F. Cavalieri acknowledges the award of an RMIT Vice Chancellor's Senior Research Fellowship. This work was performed in part at the Materials Characterisation and Fabrication Platform (MCFP) at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility (ANFF). We thank the Melbourne Cytometry Platform (Melbourne Brain Centre node) for provision of flow cytometry services. We also thank Yutian Ma for providing CaCO3 templates, Paul Brannon for assistance with the microscopy experiments, and Professor Andrew Brooks (Peter Doherty Institute for Infection and Immunity) for preparation and donation of H-2Kb-SIINFEKL tetramer. Open Access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.