P925 Deep functional assessment of the colonic mucosa-associated microbiota in Crohn’s disease and health identifies pro- and anti- inflammatory secretomes and associated bacterial consortia. The ENIGMA study

Abstract

Abstract Background Gut microbial dysbiosis and dysregulated host immune responses are key factors implicated in Crohn’s disease pathogenesis. However, the nexus between the mucosa-associated microbiota (MAM) and epithelial cell immune response(s) remains poorly understood. Here, we used in vitro cultures of LS174T human epithelial cells to examine the inflammatory tenor of the secretomes from colonic MAM recovered from healthy and Crohn’s disease subjects in Hong Kong (where Crohn’s disease incidence is low but increasing) and Australia (globally high incidence). Methods B..