Metagenomic Characterisation of The Colonic Mucosa-Associated Microbiota Reveals Novel Microbes and Communities in Health and Crohn's disease. The ENIGMA study.

Abstract

Abstract Background Deep functional understanding of the mucosa-associated microbiota (MAM) in Crohn’s disease and health by DNA sequencing approaches is restricted by the dominance of human DNA in samples. We established an ex vivo combination of tissue preservation, microbe culture, and metagenomic sequencing to overcome this knowledge gap1 and applied this approach to characterise the colonic MAM from two populations with different Crohn’s disease epidemiology (Hong Kong and Australia) and in comparison to healthy subjects. Methods Biopsies (right colon) were cryopreserv..