Journal article
Calcium Imaging and the Curse of Negativity
G Vanwalleghem, L Constantin, EK Scott
Frontiers in Neural Circuits | FRONTIERS MEDIA SA | Published : 2021
Open access
Abstract
The imaging of neuronal activity using calcium indicators has become a staple of modern neuroscience. However, without ground truths, there is a real risk of missing a significant portion of the real responses. Here, we show that a common assumption, the non-negativity of the neuronal responses as detected by calcium indicators, biases all levels of the frequently used analytical methods for these data. From the extraction of meaningful fluorescence changes to spike inference and the analysis of inferred spikes, each step risks missing real responses because of the assumption of non-negativity. We first show that negative deviations from baseline can exist in calcium imaging of neuronal acti..
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Grants
Awarded by National Institutes of Health
Funding Acknowledgements
Support was provided by NHMRC Project Grants APP1066887 and APP1165173, a Simons Foundation Pilot Award (399432), a Simons Foundation Research Award (625793), and two ARC Discovery Project Grants (DP140102036 and DP110103612) to ES, and the Australian National Fabrication Facility (ANFF), QLD node. The research reported in this publication was supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health under Award Number R01NS118406 to ES. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. GV was supported by an EMBO Long-term Fellowship.